Purified Platelet Phospholipids and Blood Coagulation

Purified platelet phospholipids and blood coagulation.

By JOHN H. FERGUSON, AARON J. MARCUS AND A. JEAN ROBINSON P LATELET PHOSPHATIDYLSERINE ( PS ) and phosphatidylethanolamine (PE) have been successfully isolated and purified1”8 by applying methods worked out by Rouser and colleagues4 for the corresponding brain phospholipids. With the modern evolution5t of clotting tests, applicable to research on the role of phosphatides7’8 in blood coagulation...

Platelet activation and blood coagulation.

Platelet activation and blood coagulation are complementary, mutually dependent processes in haemostasis and thrombosis. Platelets interact with several coagulation factors, while the coagulation product thrombin is a potent platelet-activating agonist. Activated platelets come in a procoagulant state after a prolonged elevation in cytosolic [Ca2+]i. Such platelets, e.g. when adhering to collag...

Platelet membrane involvement in blood coagulation.

Intact platelets do not show procoagulant phospholipids on their exterior. These phospholipids are located at the inside leaf of the bilayer membrane. They become available by (a) disrupture of the platelets (mechanical, osmotical etc.), (b) by a mechanism specific for the platelets, that we call the membrane flip-flop. Membrane flip-flop translocates procoagulant phospholipids (mainly phosphat...

Contribution of Different Types of Phospholipids in Blood Coagulation

In case of an injury platelets become activated and proper interaction of blood clotting proteins with membranes of activated platelets is essential for maintaining a balanced hemostasis. Hemophilia and thrombosis are the two extremes of an imbalanced hemostasis where patients with hemophilia suffer from severe bleeding and those with thrombosis from unnecessary blood clots that block normal fl...

The adsorption of coagulation factors onto phospholipids. Its role in the reaction mechanism of blood coagulation.